Journal:

Article Title: Potent Glycan Inhibitors of Myelin-associated Glycoprotein Enhance Axon Outgrowth in Vitro *

doi: 10.1074/jbc.M500250200

Figure Lengend Snippet: Rat cerebellar granule neurons were cultured on control substrata and on the same substrata adsorbed with a mild detergent extract of myelin containing MAG. As indicated, monovalent sialosides were added to control and experimental cultures 45 min after plating. After 48 h in culture, the cells were fixed and stained with anti-GAP-43 mAb. Neurite outgrowth was quantified using Metamorph image analysis software (4), and data were expressed as axon outgrowth inhibition (experimental substratum compared with matched control substratum). Values are the mean from an average of 6–7 independent wells, shown as mean ± S.E. Statistical analyses were performed using Student’s t test: †, p < 0.02; *, p < 0.001.

Article Snippet: Axon outgrowth inhibition was determined using an objective semi-automated morphometric analysis method using Metamorph image analysis software (Universal Imaging Systems, Downington, PA) as described previously ( 4 ).

Techniques: Cell Culture, Control, Staining, Software, Inhibition

Journal:

Article Title: Potent Glycan Inhibitors of Myelin-associated Glycoprotein Enhance Axon Outgrowth in Vitro *

doi: 10.1074/jbc.M500250200

Figure Lengend Snippet: Comparison of MAG binding affinities and reversal of axon outgrowth inhibition by sialoglycans

Article Snippet: Axon outgrowth inhibition was determined using an objective semi-automated morphometric analysis method using Metamorph image analysis software (Universal Imaging Systems, Downington, PA) as described previously ( 4 ).

Techniques: Comparison, Binding Assay, Inhibition